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MULTISPECTRAL MICROSCOPY & GONIOMETRY

When employing optical characterization of human tissue it is important to obtain proper characteristics of all its constituents that coexist in a complex fashion. This requires that each constituent is isolated and measured independently to determine how it reacts to different wavelengths of light (primarily absorption). Besides the hyperspectral camera, we have a multispectral microscope which employs a range of LEDs in a broad spectral range (350 nm – 940 nm) that can independently illuminate a sample from which absorption and scattering characteristics can be determined.  Here we can obtain an even higher spatial resolution than the hyperspectral camera and can therefore potentially provide information about the absorbing chromophores that may exist in for example various cancer tissue.

The goniometer employs the same light sources as the multispectral microscope, but has an extended capability to measure the scattering properties of a sample by determining the preferred angles light choses to scatter into. Therefore, these two tools working in conjunction become important when characterizing the optical properties of human tissue, which in turn lays a foundation for proper interpretation of signals generated by other methods, such as photoacoustic imaging.

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